Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay
作者: Xiaodong LyuXianwei WangLina ZhangZhenzhu ChenYu ZhaoJieying HuRuihua FanYongping Song
作者单位: 1Zhengzhou University
2the Affiliated Cancer Hospital of Zhengzhou University; Henan Cancer Hospital
刊名: Diagnostic Pathology, 2017, Vol.12 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s13000-017-0634-3
关键词: LeukemiaF-qRT-PCRAMLMDSCLLALLChromosomal rearrangementGene fusion
英文摘要: Fusion genes generated from chromosomal translocation play an important role in hematological malignancies. Detection of fusion genes currently employ use of either conventional RT-PCR methods or fluorescent in situ hybridization (FISH), where both methods involve tedious methodologies and require prior characterization of chromosomal translocation events as determined by cytogenetic analysis. In this study, we describe a real-time quantitative reverse transcription PCR (qRT-PCR)-based multi-fusion gene screening method with the capacity to detect 22 fusion genes commonly found in leukemia. This method does not require pre-characterization of gene translocation events, thereby facilitating immediate diagnosis and therapeutic management.
原始语种摘要: Fusion genes generated from chromosomal translocation play an important role in hematological malignancies. Detection of fusion genes currently employ use of either conventional RT-PCR methods or fluorescent in situ hybridization (FISH), where both methods involve tedious methodologies and require prior characterization of chromosomal translocation events as determined by cytogenetic analysis. In this study, we describe a real-time quantitative reverse transcription PCR (qRT-PCR)-based multi-fusion gene screening method with the capacity to detect 22 fusion genes commonly found in leukemia. This method does not require pre-characterization of gene translocation events, thereby facilitating immediate diagnosis and therapeutic management.
全文获取路径: Springer Nature  (合作)
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影响因子:1.85 (2012)

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关键词翻译
关键词翻译
  • leukemic 白血病的
  • multiplex 多倍仪
  • PCR Page Control Register
  • fusion 融解
  • hematological 血液学的
  • translocation 移位
  • CLL Critical Load Line
  • leukemia 白血病
  • chromosomal 染色体的
  • AML Micro Switch