In vitro and in vivo analyses of the role of the carboxysomal β-type carbonic anhydrase of the cyanobacterium Synechococcus elongatus in carboxylation of ribulose-1,5-bisphosphate
作者: Takashi NishimuraOsamu YamaguchiNobuyuki TakataniShin-ichi MaedaTatsuo Omata
作者单位: 1Graduate School of Bioagricultural Sciences, Nagoya University
2Japan Science and Technology Agency, CREST
刊名: Photosynthesis Research, 2014, Vol.121 (2-3), pp.151-157
来源数据库: Springer Nature Journal
DOI: 10.1007/s11120-014-9986-7
关键词: Carbonic anhydraseCarboxysomeCyanobacteriaCO 2 fixationRubiscoSynechococcus elongatus
英文摘要: Abstract(#br)The carboxylase activities of crude carboxysome preparations obtained from the wild-type Synechococcus elongatus strain PCC 7942 strain and the mutant defective in the carboxysomal carbonic anhydrase (CA) were compared. The carboxylation reaction required high concentrations of bicarbonate and was not even saturated at 50 mM bicarbonate. With the initial concentrations of 50 mM and 25 mM for bicarbonate and ribulose-1,5-bisphosphate (RuBP), respectively, the initial rate of RuBP carboxylation by the mutant carboxysome (0.22 μmol mg −1 protein min −1 ) was only 30 % of that observed for the wild-type carboxysomes (0.71 μmol mg −1 protein min −1 ), indicating the importance of the presence of CA in efficient catalysis by ribulose-1,5-bisphosphate carboxylase/oxygenase...
全文获取路径: Springer Nature  (合作)
影响因子:3.15 (2012)

  • ribulose 核酮糖
  • carboxylation 羧化
  • Synechococcus synechococcus属
  • anhydrase 脱水酶
  • carbonic 碳的
  • vitro 体外