Stabilization of Cellular RNA in Blood During Storage at Room Temperature: A Comparison of Cell-Free RNA BCT ® with K3EDTA Tubes
作者: Kausik DasSheila E. NortonJodi R. AltGary D. KrzyzanowskiThomas L. WilliamsM. Rohan Fernando
作者单位: 1Research and Development Division, Streck Inc.
2Methodist Hospital Laboratory
刊名: Molecular Diagnosis & Therapy, 2014, Vol.18 (6), pp.647-653
来源数据库: Springer Nature Journal
DOI: 10.1007/s40291-014-0118-z
英文摘要: Abstract(#br) Background(#br)Messenger RNA (mRNA) expression levels in blood cells are important in disease diagnosis, prognosis and biomarker discovery research. Accurate measurements of intracellular mRNA levels in blood cells depend upon several pre-analytical factors, including delays in RNA extraction from blood after phlebotomy. Dramatic changes in mRNA expression levels caused by delays in blood sample processing may render such samples unsuitable for gene expression analysis.(#br) Objectives(#br)This study was conducted to evaluate a blood collection tube, cell-free RNA-BCT ® (RNA-BCT), for its ability to stabilize mRNA expression level in blood cells post-phlebotomy using indicator mRNAs in reverse transcription quantitative real-time PCR (RT-qPCR) assays.(#br) Methods(#br)Blood...
全文获取路径: Springer Nature  (合作)
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影响因子:1.692 (2012)

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关键词翻译
关键词翻译
  • RNA ROYAL NEPAL AIRLINES CORP.
  • Blood 
  • Temperature 温度
  • EDTA Ethylenediamine Tetraacetate