Protective immunity against Rickettsia heilongjiangensis in a C3H/HeN mouse model mediated by outer membrane protein B-pulsed dendritic cells
作者: YanFen MengXiaoLu XiongYong QiChangSong DuanWenPing GongJun JiaoBoHai Wen
作者单位: 1State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
刊名: Science China Life Sciences, 2015, Vol.58 (3), pp.287-296
来源数据库: Springer Nature Journal
DOI: 10.1007/s11427-014-4720-4
关键词: Rickettsia heilongjiangensisdendritic cellsouter membrane proteinprotective immunity
原始语种摘要: Abstract(#br) Rickettsia heilongjiangensis is an obligate intracellular bacterium that causes Far-Eastern tick-borne spotted fever. Outer membrane protein B (OmpB) is an important surface protein antigen of rickettsiae. In the present study, the ompB gene of R. heilongjiangensis was divided into four fragments, resulting in four recombinant proteins (OmpB-p1, OmpB-p2, OmpB-p3, and OmpB-p4). Each OmpB was used in vitro to stimulate murine bone marrow-derived dendritic cells (BMDCs) of C3H/HeN mice, and the OmpB-pulsed BMDCs were transferred to naïve C3H/HeN mice. On day 14 post-transfer of BMDCs, the mice were challenged with R. heilongjiangensis and the rickettsial loads in the mice were quantitatively determined on day 7 post-challenge. Mice receiving BMDCs pulsed with OmpB-p2, OmpB-p3,...
全文获取路径: Springer Nature  (合作)
影响因子:2.024 (2011)

  • dendritic 师状的
  • immunity 免疫
  • protein 蛋白质
  • against 反对
  • mouse 小鼠
  • cells 麻风细胞
  • membrane 
  • outer 外部的
  • pulsed 脉冲
  • model 模型