Is hematoxylin-eosin staining in rectal mucosal and submucosal biopsies still useful for the diagnosis of Hirschsprung disease?
作者: Suellen SerafiniMaria Mercês SantosAna Cristina Aoun TannuriMaria Claudia Nogueira ZerbiniMaria Cecília de Mendonça CoelhoJosiane de Oliveira GonçalvesUenis Tannuri
作者单位: 1University of Sao Paulo Medical School
2Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo
刊名: Diagnostic Pathology, 2017, Vol.12 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s13000-017-0673-9
关键词: Hirschsprung’s diseaseRectal biopsyAcetylcholinesteraseHematoxylin-eosinCalretinin
英文摘要: Hematoxylin-eosin (HE) staining of a full-thickness rectal wall fragment is classically used for the diagnosis of Hirschsprung disease (HD). However, this technique requires large fragments for a better diagnosis. Additionally, the histochemical and immunohistochemical methods of staining small fragments of rectal mucosal and submucosal biopsies are not available in all centers. Therefore, the possibility of diagnosing HD through HE staining in these biopsies could be a valuable alternative for centers that do not have more specific techniques. The objectives of the current investigation were to evaluate the concordance of the results obtained by HE staining and the calretinin method with acetylcholinesterase (AChE) activity in fragments of mucosa and submucosa in the diagnosis of HD.
原始语种摘要: Hematoxylin-eosin (HE) staining of a full-thickness rectal wall fragment is classically used for the diagnosis of Hirschsprung disease (HD). However, this technique requires large fragments for a better diagnosis. Additionally, the histochemical and immunohistochemical methods of staining small fragments of rectal mucosal and submucosal biopsies are not available in all centers. Therefore, the possibility of diagnosing HD through HE staining in these biopsies could be a valuable alternative for centers that do not have more specific techniques. The objectives of the current investigation were to evaluate the concordance of the results obtained by HE staining and the calretinin method with acetylcholinesterase (AChE) activity in fragments of mucosa and submucosa in the diagnosis of HD.
全文获取路径: Springer Nature  (合作)
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来源刊物:
影响因子:1.85 (2012)

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关键词翻译
关键词翻译
  • submucosal 粘膜下层的
  • rectal 直肠的
  • staining 染色
  • diagnosis 特镇述
  • eosin 伊经
  • hematoxylin 苏木精
  • disease 
  • histochemical 组织化学的
  • centers 机组间距
  • concordance 整合