Changes in calpain and caspase gene expression at the mRNA level during bovine muscle satellite cell myogenesis and the correlation between the cell model and the muscle tissue
作者: W. WangY. B. YangX. Y. MaX. L. YuI. Hwang
作者单位: 1Henan University of Science and Technology
2Chonbuk National University
刊名: Russian Journal of Bioorganic Chemistry, 2017, Vol.43 (3), pp.270-277
来源数据库: Springer Nature Journal
DOI: 10.1134/S1068162017030177
关键词: Skeletal muscle satellite cellIsolationCultureCalpainsCaspasesReal-time PCR
英文摘要: The calpain proteolytic system plays a central role in cell death and cell signaling. Caspases are a family of proteases implicated in apoptosis. The objective of this study was to explore the regulation and change trend of calpains (CAPN1 and CAPN3) and caspases (caspase-3, caspase-7, and caspase-9) expression at the mRNA level in Luxi cattle skeletal muscle satellite cells during proliferation and differentiation into myotubes. We also sought to assess whether there is a relationship between the muscle satellite cell model and skeletal muscle tissue. Satellite cells were isolated from longissimus dorsi muscle from Luxi cattle and cultured in vitro. Immunofluorescence was used to characterize satellite cells. Our study was divided into three groups: stage one, satellite cells...
原始语种摘要: The calpain proteolytic system plays a central role in cell death and cell signaling. Caspases are a family of proteases implicated in apoptosis. The objective of this study was to explore the regulation and change trend of calpains (CAPN1 and CAPN3) and caspases (caspase-3, caspase-7, and caspase-9) expression at the mRNA level in Luxi cattle skeletal muscle satellite cells during proliferation and differentiation into myotubes. We also sought to assess whether there is a relationship between the muscle satellite cell model and skeletal muscle tissue. Satellite cells were isolated from longissimus dorsi muscle from Luxi cattle and cultured in vitro. Immunofluorescence was used to characterize satellite cells. Our study was divided into three groups: stage one, satellite cells...
全文获取路径: Springer Nature  (合作)
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影响因子:0.523 (2012)

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关键词翻译
关键词翻译
  • muscle 肌肉
  • myogenesis 肌生成
  • expression 表示
  • satellite 卫星
  • calpain 钙激活中性蛋白酶
  • confluence 汇零
  • skeletal 骨架
  • cells 麻风细胞
  • level 水准
  • between 在中间