An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing
作者: Parisa AminiJulia EttlinLennart OpitzElena ClementiAlexandra MalbonEnni Markkanen
作者单位: 1University of Zürich
2Functional Genomics Center Zurich, University of Zürich/ETH Zürich
3Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zürich
刊名: BMC Molecular Biology, 2017, Vol.18 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s12867-017-0099-7
关键词: FFPERNA isolationRNAsequencingLCMRT-qPCRDogCancerMammary carcinomaMammary tumour
原始语种摘要: Formalin-fixed paraffin embedded (FFPE) tissue constitutes a vast treasury of samples for biomedical research. Thus far however, extraction of RNA from FFPE tissue has proved challenging due to chemical RNA–protein crosslinking and RNA fragmentation, both of which heavily impact on RNA quantity and quality for downstream analysis. With very small sample sizes, e.g. when performing Laser-capture microdissection (LCM) to isolate specific subpopulations of cells, recovery of sufficient RNA for analysis with reverse-transcription quantitative PCR (RT-qPCR) or next-generation sequencing (NGS) becomes very cumbersome and difficult.
全文获取路径: Springer Nature  (合作)
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影响因子:2.796 (2012)

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关键词翻译
关键词翻译
  • sequencing 排序
  • RNA ROYAL NEPAL AIRLINES CORP.
  • small 小的
  • tissue 组织
  • generation 世代
  • isolation 隔离
  • cumbersome 笨重的
  • capture 捕获
  • downstream 顺聊
  • treasury 财政部