Selection of reliable reference genes for the normalisation of gene expression levels following time course LPS stimulation of murine bone marrow derived macrophages
作者: Akane TanakaJoyce ToBronwyn O’BrienSheila DonnellyMaria Lund
作者单位: 1University of Technology Sydney
刊名: BMC Immunology, 2017, Vol.18 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s12865-017-0223-y
关键词: MacrophageLipopolysaccharidePro-inflammatory responsesRT-qPCRReference gene
英文摘要: Macrophages are key players in the initiation, perpetuation and regulation of both innate and adaptive immune responses. They largely perform these roles through modulation of the expression of genes, especially those encoding cytokines. Murine bone marrow derived macrophages (BMDMs) are commonly used as a model macrophage population for the study of immune responses to pro-inflammatory stimuli, notably lipopolysaccharide (LPS), which may be pertinent to the human situation. Evaluation of the temporal responses of LPS stimulated macrophages is widely conducted via the measurement of gene expression levels by RT-qPCR. While providing a robust and sensitive measure of gene expression levels, RT-qPCR relies on the normalisation of gene expression data to a stably expressed reference gene....
原始语种摘要: Macrophages are key players in the initiation, perpetuation and regulation of both innate and adaptive immune responses. They largely perform these roles through modulation of the expression of genes, especially those encoding cytokines. Murine bone marrow derived macrophages (BMDMs) are commonly used as a model macrophage population for the study of immune responses to pro-inflammatory stimuli, notably lipopolysaccharide (LPS), which may be pertinent to the human situation. Evaluation of the temporal responses of LPS stimulated macrophages is widely conducted via the measurement of gene expression levels by RT-qPCR. While providing a robust and sensitive measure of gene expression levels, RT-qPCR relies on the normalisation of gene expression data to a stably expressed reference gene....
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影响因子:2.61 (2012)

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关键词翻译
关键词翻译
  • expression 表示
  • murine 鼠灰色
  • macrophages 巨噬细胞
  • LPS Laboratory Peripheral System
  • stimulation 激励
  • reference 基准电压源
  • derived 导生的
  • following 追踪
  • immune 免疫的
  • cytokines 细胞浆移动