Production of Monoclonal Antibodies against Human Trefoil Factor 3 and Development of a Modified-Sandwich ELISA for Detection of Trefoil Factor 3 Homodimer in Saliva
作者: Saichit KhummuangWaraporn PhanphromWitida LaopajonWatchara KasinrerkPonlatham ChaiyaritSupansa Pata
作者单位: 1Faculty of Associated Medical Sciences, Chiang Mai University
2Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences, Chiang Mai University
3Faculty of Dentistry, Khon Kaen University
4Research Group of Chronic Inflammatory Oral Diseases and Systemic Diseases Associated with
刊名: Biological Procedures Online, 2017, Vol.19 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s12575-017-0064-3
关键词: Monoclonal AntibodyModified-sandwich ELISATrefoil FactorSalivaOral DiseasesHomodimeric Peptides
英文摘要: Human trefoil factor (TFF) peptides consist of three members: TFF1, TFF2 and TFF3. TFF3 is the most abundant TFF peptide in saliva. TFF3 homodimer was suggested to be involved in apoptosis inhibition and malignancy. Determination of TFF3 homodimer expression profiles in saliva may lead to new information about oral biology and diseases. The objective of this study was to generate monoclonal antibodies (mAbs) against TFF3 and apply the produced mAbs for the establishment of ELISA for quantification of dimeric TFF3 in saliva.
原始语种摘要: Human trefoil factor (TFF) peptides consist of three members: TFF1, TFF2 and TFF3. TFF3 is the most abundant TFF peptide in saliva. TFF3 homodimer was suggested to be involved in apoptosis inhibition and malignancy. Determination of TFF3 homodimer expression profiles in saliva may lead to new information about oral biology and diseases. The objective of this study was to generate monoclonal antibodies (mAbs) against TFF3 and apply the produced mAbs for the establishment of ELISA for quantification of dimeric TFF3 in saliva.
全文获取路径: Springer Nature  (合作)
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影响因子:0.95 (2012)

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关键词翻译
关键词翻译
  • homodimer 同型二聚体
  • ELISA 酶联免疫吸收分析
  • against 反对
  • monoclonal 单细胞系的
  • sandwich 多层结构材料
  • malignancy 恶性
  • peptide 
  • involved 涉及
  • expression 表示
  • establishment 确立