Overexpression of calmodulin gene fragment from Antarctic notothenioid fish improves chilling tolerance in Nicotiana benthamiana
作者: T. J. ZhangL. J. PanQ. HuangL. H. ZhuN. YangC. L. PengL. B. Chen
作者单位: 1South China Normal University
2Shanghai Ocean University
刊名: Photosynthetica, 2017, Vol.55 (4), pp.630-637
来源数据库: Springer Nature Journal
DOI: 10.1007/s11099-016-0682-z
关键词: Calcium-binding proteinChilling stressChlorophyll fluorescenceElectrolyte leakageTransgenic plants
英文摘要: Calmodulin (CaM) is a highly conserved calcium sensor protein associated with chilling tolerance in living organisms. It has four EF-hand domains for binding of four Ca2+, two of them located in the N-terminus, and the other two in the C-terminus. A notothenioid CaM gene fragment ( CaMm ), which only codes for N-terminus of CaM (with two EF-hand domains), was introduced into Nicotiana benthamiana . Effects of its overexpression on chilling tolerance in plants were explored. During 4◦C or 0◦C chilling treatment, both CaMm and CaM transgenic plants showed higher PSII maximum quantum yield, actual quantum yield, and soluble protein content, lower electrolyte leakage and malondialdehyde content than that of the control. The changes in these physiological indices were comparable...
原始语种摘要: Calmodulin (CaM) is a highly conserved calcium sensor protein associated with chilling tolerance in living organisms. It has four EF-hand domains for binding of four Ca2+, two of them located in the N-terminus, and the other two in the C-terminus. A notothenioid CaM gene fragment ( CaMm ), which only codes for N-terminus of CaM (with two EF-hand domains), was introduced into Nicotiana benthamiana . Effects of its overexpression on chilling tolerance in plants were explored. During 4◦C or 0◦C chilling treatment, both CaMm and CaM transgenic plants showed higher PSII maximum quantum yield, actual quantum yield, and soluble protein content, lower electrolyte leakage and malondialdehyde content than that of the control. The changes in these physiological indices were comparable...
全文获取路径: Springer Nature  (合作)
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影响因子:0.862 (2012)

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关键词翻译
关键词翻译
  • fragment 碎屑
  • chilling 冷硬
  • terminus 末端
  • functional 功能的
  • tolerance 耐性
  • protein 蛋白质
  • likely 大概
  • binding 装订
  • lower 降下
  • involved 涉及