A sensitive colorimetric assay system for nucleic acid detection based on isothermal signal amplification technology
作者: Bo HuJing GuoYing XuHua WeiGuojie ZhaoYifu Guan
作者单位: 1China Medical University
刊名: Analytical and Bioanalytical Chemistry, 2017, Vol.409 (20), pp.4819-4825
来源数据库: Springer Nature Journal
DOI: 10.1007/s00216-017-0425-4
关键词: Colorimetric sensingRolling circle amplificationGold nanoparticlesOligonucleotidesMicroRNA
原始语种摘要: Rapid and accurate detection of microRNAs in biological systems is of great importance. Here, we report the development of a visual colorimetric assay which possesses the high amplification capabilities and high selectivity of the rolling circle amplification (RCA) method and the simplicity and convenience of gold nanoparticles used as a signal indicator. The designed padlock probe recognizes the target miRNA and is circularized, and then acts as the template to extend the target miRNA into a long single-stranded nucleotide chain of many tandem repeats of nucleotide sequences. Next, the RCA product is hybridized with oligonucleotides tagged onto gold nanoparticles. This interaction leads to the aggregation of gold nanoparticles, and the color of the system changes from wine red to dark...
全文获取路径: Springer Nature  (合作)
影响因子:3.659 (2012)

  • colorimetric 比色的
  • amplification 放大
  • sensitive 灵敏的
  • nucleotide 核苷酸
  • assay 试金
  • detection 探测
  • stranded 绞合的搁浅的
  • target 目标
  • system 
  • padlock 挂锁