Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress
作者: Xun TangNing ZhangHuaijun SiAlejandro Calderón-Urrea
作者单位: 1Gansu Agricultural University
2California State University
刊名: Plant Methods, 2017, Vol.13 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s13007-017-0238-7
关键词: Reference geneDrought stressOsmotic stressPotato
原始语种摘要: Real-time quantitative PCR (RT-qPCR) is the most commonly used method for accurately detecting gene expression patterns. As part of RT-qPCR analysis, normalization of the data requires internal control gene(s) that display uniform expression under different biological conditions. However, no invariable internal control gene exists, and therefore more than one reference gene is needed to normalize RT-qPCR results. Identification of stable reference genes in potato will improve assay accuracy for selecting stress-tolerance genes and identifying molecular mechanisms conferring stress tolerance in this species.
全文获取路径: Springer Nature  (合作)
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影响因子:2.667 (2012)

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关键词翻译
关键词翻译
  • reference 基准电压源
  • stress 应力
  • control 控制
  • analysis 分析
  • potato 马铃薯
  • therefore 因此
  • Selection 分选
  • normalize 使规格化
  • expression 表示
  • normalization 正规化