Evaluation of six serological ELISA kits available in Italy as screening tests for equine infectious anaemia surveillance
作者: Roberto NardiniGian Luca AutorinoCharles J. IsselR. Frank CookIda RicciRaffaele FrontosoFrancesca RosoneMaria Teresa Scicluna
作者单位: 1Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”
2University of Kentucky
刊名: BMC Veterinary Research, 2017, Vol.13 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s12917-017-1007-6
关键词: Equine infectious anaemiaELISAAGIDComparisonCommercial assaysIn-house assaysSurveillance
英文摘要: ELISAs are known to have a higher diagnostic sensitivity than the agar gel immunodiffusion (AGID) when employed for serological diagnosis of equine infectious anaemia (EIA). For this purpose, an “in-house” and five commercial ELISAs available in Italy were assessed by the National Reference Centre for EIA for their analytic specificity (Sp); precocity, defined as capability of detecting first antibodies produced during a new infection; precision based on repeatability and reproducibility, estimated from the coefficient of variation (CV); accuracy, estimated from multiple K and relative Sp and sensitivity (Se). Two serum panels, positive for non-equine retroviruses and the most frequent equine viruses, were employed to measure analytic Sp. ELISA precocity was also compared to that of one...
原始语种摘要: ELISAs are known to have a higher diagnostic sensitivity than the agar gel immunodiffusion (AGID) when employed for serological diagnosis of equine infectious anaemia (EIA). For this purpose, an “in-house” and five commercial ELISAs available in Italy were assessed by the National Reference Centre for EIA for their analytic specificity (Sp); precocity, defined as capability of detecting first antibodies produced during a new infection; precision based on repeatability and reproducibility, estimated from the coefficient of variation (CV); accuracy, estimated from multiple K and relative Sp and sensitivity (Se). Two serum panels, positive for non-equine retroviruses and the most frequent equine viruses, were employed to measure analytic Sp. ELISA precocity was also compared to that of one...
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影响因子:1.861 (2012)

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关键词翻译
关键词翻译
  • equine 马的
  • infectious 传染性的
  • serological 血清学的
  • anaemia 贫血
  • available 可供应的
  • precocity 早熟性
  • ELISA 酶联免疫吸收分析
  • panel 
  • positive 阳片
  • employed 就业