Tracking the best reference genes for RT-qPCR data normalization in filamentous fungi
作者: Agustina LlanosJean Marie FrançoisJean-Luc Parrou
作者单位: 1Université de Toulouse; INSA, UPS, INP; LISBP
2INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés
3CNRS, UMR5504
4Cinabio-Adisseo France S.A.S.
刊名: BMC Genomics, 2015, Vol.16 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s12864-015-1224-y
关键词: Filamentous fungiTalaromycesRNA-seqRT-qPCRReference genesNormalizationGene expression
英文摘要: Abstract(#br) Background(#br)A critical step in the RT-qPCR workflow for studying gene expression is data normalization, one of the strategies being the use of reference genes. This study aimed to identify and validate a selection of reference genes for relative quantification in Talaromyces versatilis , a relevant industrial filamentous fungus. Beyond T. versatilis , this study also aimed to propose reference genes that are applicable more widely for RT-qPCR data normalization in filamentous fungi.(#br) Results(#br)A selection of stable, potential reference genes was carried out in silico from RNA-seq based transcriptomic data obtained from T. versatilis . A dozen functionally unrelated candidate genes were analysed by RT-qPCR assays over more than 30 relevant culture conditions. By...
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影响因子:4.397 (2012)

  • normalization 正规化
  • Tracking 追踪
  • filamentous 丝状的
  • reference 基准电压源
  • fungi 真菌