Artificial miRNA inhibition of phosphoenolpyruvate carboxylase increases fatty acid production in a green microalga Chlamydomonas reinhardtii
作者: Chaogang WangXi ChenHui LiJiangxin WangZhangli Hu
作者单位: 1Shenzhen University
刊名: Biotechnology for Biofuels, 2017, Vol.10 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s13068-017-0779-z
关键词: Chlamydomonas reinhardtiiLipidsBiodieselAmiRNACrPEPC1CrPEPC2
原始语种摘要: Nutrient limitation, such as nitrogen depletion, is the most widely used method for improving microalgae fatty acid production; however, these harsh conditions also inhibit algal growth significantly and even kill cells at all. To avoid these problems, we used artificial microRNA (amiRNA) technology as a useful tool to manipulate metabolic pathways to increase fatty acid contents effectively in the green microalga Chlamydomonas reinhardtii . We down-regulated the expression of phosphoenolpyruvate carboxylase (PEPC), which catalyzes the formation of oxaloacetate from phosphoenolpyruvate and regulates carbon flux.
全文获取路径: Springer Nature  (合作)
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影响因子:5.552 (2012)

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关键词翻译
关键词翻译
  • carboxylase 羧化酶
  • Chlamydomonas 杆菌属
  • green 绿色
  • fatty 脂肪的
  • improving 改进
  • conditions 条件式
  • oxaloacetate 草酰乙酸盐
  • significantly 较大地
  • contents 目次
  • increase 增加