Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement
作者: Mayu YasunagaYasuko FujitaRumiko SaitoMitsuo OshimuraYoshihiro Nakajima
作者单位: 1Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
2Tohoku University
3Tottori University
刊名: BMC Biotechnology, 2017, Vol.17 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s12896-017-0374-1
关键词: Luciferase3D cultureArtificial chromosome vectorLong-term monitoringCytotoxicityEmerald Luc (ELuc)HepatocytesBioluminescence
英文摘要: Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated treatment of the spheroids with a test object is possible. Generally, in the repeated treatment test to assess cytotoxicity in the spheroids, ATP assay, colorimetric measurement using pigments or high-content imaging analysis is performed. However, continuous assessment of cytotoxicity in the same spheroids using the above assays or analysis is impossible because the spheroids must be disrupted or killed. To overcome this technical limitation, we constructed a simple monitoring system in which cytotoxicity in the spheroids can be continuously monitored by nondestructive...
原始语种摘要: Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated treatment of the spheroids with a test object is possible. Generally, in the repeated treatment test to assess cytotoxicity in the spheroids, ATP assay, colorimetric measurement using pigments or high-content imaging analysis is performed. However, continuous assessment of cytotoxicity in the same spheroids using the above assays or analysis is impossible because the spheroids must be disrupted or killed. To overcome this technical limitation, we constructed a simple monitoring system in which cytotoxicity in the spheroids can be continuously monitored by nondestructive...
全文获取路径: Springer Nature  (合作)
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来源刊物:
影响因子:2.165 (2012)

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关键词翻译
关键词翻译
  • luciferase 荧光素酶
  • measurement 测量
  • expressing 压榨
  • monitoring 监视
  • cytotoxicity 细胞毒性
  • repeated 重复的
  • nondestructive 不破损的
  • possible 可能的
  • disrupted 断线状
  • resemble 类似