A simple method for isolation and construction of markerless cyanobacterial mutants defective in acyl-acyl carrier protein synthetase
作者: Kouji KojimaSumie KetaKazuma UesakaAkihiro KatoNobuyuki TakataniKunio IharaTatsuo OmataMakiko Aichi
作者单位: 1Chubu University
2Japan Science and Technology Agency, CREST
3Nagoya University
刊名: Applied Microbiology and Biotechnology, 2016, Vol.100 (23), pp.10107-10113
来源数据库: Springer Nature Journal
DOI: 10.1007/s00253-016-7850-8
关键词: Acyl-ACP synthetaseBiofuelCyanobacteriaFree-fatty acidCounter selectionSensitivity to FFAs
原始语种摘要: Abstract(#br)Cyanobacterial mutants defective in acyl-acyl carrier protein synthetase (Aas) secrete free fatty acids (FFAs) into the external medium and hence have been used for the studies aimed at photosynthetic production of biofuels. While the wild-type strain of Synechocystis sp. PCC 6803 is highly sensitive to exogenously added linolenic acid, mutants defective in the aas gene are known to be resistant to the externally provided fatty acid. In this study, the wild-type Synechocystis cells were shown to be sensitive to lauric, oleic, and linoleic acids as well, and the resistance to these fatty acids was shown to be enhanced by inactivation of the aas gene. On the basis of these observations, we developed an efficient method to isolate aas -deficient mutants from cultures of...
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来源刊物:
影响因子:3.689 (2012)

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关键词翻译
关键词翻译
  • synthetase 合成酶
  • defective 有缺陷的
  • protein 蛋白质
  • isolation 隔离
  • construction 构造
  • carrier 载体
  • simple 单纯的
  • method 方法