Determination of N -acetylneuraminic acid in poultry eggs by ultra performance liquid chromatography–tandem mass spectrometry
作者: Y. LiW. WangX. L. GouN. LinS. F. LeN. DuH. YanJ. H. Zhang
作者单位: 1Beijing University of Technology
2Beijing Center for Physical and Chemical Analysis
刊名: Journal of Analytical Chemistry, 2017, Vol.72 (8), pp.886-889
来源数据库: Springer Nature Journal
DOI: 10.1134/S1061934817080081
关键词: N -acetylneuraminic acidUltra performance liquid chromatography–tandem mass spectrometryPoultry eggs
原始语种摘要: N -acetylneuraminic acid is an important member of sialic acids which is a family of nine-carbon carboxylated sugars most frequently found in terminal position in glycoconjugates. It was widely distributed in different portions of organisms. In this study, a method of ultra performance liquid chromatography–tandem mass spectrometry was developed for the determination of N -acetylneuraminic acid in poultry eggs. The N -acetylneuraminic acid was freed by heating the materials in 0.1 M H2SO4 at 80°C for 2 h. Then, chloroform reagent was used to eliminate phospholipids, cholesterol and other organic impurities, meanwhile, it was able to precipitate protein. In the cleanup step, C18 solid phase extraction columns were used to purify the matrix. Determination of...
全文获取路径: Springer Nature  (合作)
影响因子:0.616 (2012)

  • liquid 液体
  • chromatography 色层分离法
  • performance 性能
  • tandem 串联机
  • spectrometry 光谱测定
  • acetonitrile 乙腈
  • precipitate 沉淀物
  • chloroform 氯仿
  • organic 有机的
  • elution 洗提