Selective Energy Transfer Between Quantum Dots and Gold Nanoparticles for Detection of Multiple Mutations in Epidermal Growth Factor Receptor
作者: Hosub LeeAhreum KimTaegyeong KangSang-Woo JooSo Yeong LeeKyong-Ah YoonKangtaek Lee
作者单位: 1Department of Chemical and Biomolecular Engineering , Yonsei University , Seoul , Republic of Korea
2Department of Chemistry , Soongsil University , Seoul , Republic of Korea
3Laboratory of Veterinary Pharmacology, Seoul National University , Seoul , Republic of Korea
4National Cancer Center , Goyang , Gyeonggi , Republic of Korea
刊名: Analytical Letters, 2012, Vol.45 (18), pp.2707-2716
来源数据库: Taylor & Francis Journal
DOI: 10.1080/00032719.2012.702177
关键词: EGFREnergy transferFluorescence quenchingGold nanoparticlesMultiple mutationQuantum dots
原始语种摘要: Selective energy transfer between quantum dots and gold nanoparticles was used to simultaneously detect mutations in the epidermal growth factor receptor (EGFR) gene. We functionalized the surface of gold nanoparticles and green and red-emitting quantum dots using four different probe DNAs that were designed to be a perfect complementary to an in-frame deletion mutation in exon 19 or L858 R point mutation in exon 21 of EGFR. We found that the presence of the deletion mutation in exon 19 in target oligonucleotides caused fluorescence quenching at 525 nm due to energy transfer from green-emitting quantum dots to gold nanoparticles, whereas point mutation in exon 21 resulted in quenching at 620 nm due to energy transfer from red-emitting quantum dots to gold nanoparticles. This method could...
全文获取路径: Taylor & Francis  (合作)
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影响因子:0.965 (2012)

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关键词翻译
关键词翻译
  • Transfer 转运牌汽车
  • Growth 生长
  • Energy 能量