Adaptive laboratory evolution of Klebsiella pneumoniae for improving 2,3-butanediol production
作者: Hongbiao LiGenlin ZhangYanyan Dang
作者单位: 1Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University , Shihezi , China
刊名: Bioengineered, 2016, Vol.7 (6), pp.432-438
来源数据库: Taylor & Francis Journal
DOI: 10.1080/21655979.2016.1199304
关键词: Adaptive laboratory evolutionCotton stalkFermentationKlebsiella pneumoniae23-butanediol
原始语种摘要: ABSTRACT(#br)Microbial production of 2,3-butanediol is limited by the toxic components in the lignocellulose hydrolysate. To improve the 2,3-butanediol production via Klebsiella pneumoniae from cotton stalk hydrolysate, a method coupling a high tolerance of strain and detoxification of the hydrolysate was thus investigated in this study. The strain tolerance of K. pneumoniae to the cotton stalk hydrolysate was improved via an adaptive laboratory evolution, which involved a stepwise increase in the hydrolysate concentration in the medium. Compared with the initial strain, the resulting strain increased the biomass 3.2-fold in a medium of 20 g/L hydrolysate and produced 10.45 g/L of 2,3-butanediol at an optimal concentration of 60 g/L hydrolysate. After detoxification of cotton stalk...
全文获取路径: Taylor & Francis  (合作)

  • butanediol 丁二醇
  • laboratory 实验室
  • evolution 进化
  • production 生产
  • hydrolysate 水解产物
  • stalk 晶体管管座
  • cotton 
  • concentration 浓度
  • stepwise 步进式
  • Cotton 棉花