Effect of leukaemia inhibitory factor and different types of feeder layers on growth and pluripotent nature of embryonic stem cells from in vitro produced goat (Capra hircus) blastocysts
作者: Arun Kumar DeDhruba MalakarRahul DuttaShailendra SahuManoj Kumar Jena
作者单位: 1Animal Biotechnology Centre , National Dairy Research Institute , Karnal , Haryana , India
刊名: Journal of Applied Animal Research, 2011, Vol.39 (4), pp.311-316
来源数据库: Taylor & Francis Journal
DOI: 10.1080/09712119.2011.607898
关键词: embryonic stem cellsfeeder layergoatleukaemia inhibitory factor
原始语种摘要: Feeder layer and leukaemia inhibitory factor (LIF) play an important role in maintaining the embryonic stem cells in undifferentiated state and help in their unlimited proliferation. The aim of the present investigation was to see the effect of LIF and different types of feeder layers on growth and pluripotent nature of goat embryonic stem cells. Goat blastocysts were produced in vitro by standard methods of in vitro maturation (IVM), in vitro fertilisation (IVF) and in vitro culture (IVC) techniques. Inner cell mass (ICM) cells were isolated mechanically and cultured in embryonic stem (ES) cell medium on inactivated feeder layer supplemented with or without LIF. When ICM cells were cultured on goat fibroblast feeder layer in ES cell culture medium supplemented with LIF they maintained...
全文获取路径: Taylor & Francis  (合作)
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影响因子:0.123 (2012)

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