REVIEW: LIQUID CHROMATOGRAPHY—POST-COLUMN DERIVATIZATION FOR AMINO ACID ANALYSIS: STRATEGIES, INSTRUMENTATION, AND APPLICATIONS
作者: Pantelis G. Rigas
作者单位: 1Biotoxins and Analytical Chemistry Laboratory, Department of Fisheries and Aquaculture Technology , Alexander Technological Educational Institute of Thessaloniki , N. Moudania , Greece
刊名: Instrumentation Science & Technology, 2012, Vol.40 (2-3), pp.161-193
来源数据库: Taylor & Francis Journal
DOI: 10.1080/10739149.2011.651669
关键词: amino acid analysision-exchange chromatographypost-column derivatization
原始语种摘要: Amino acid analysis (AAA) is widely used for determining the composition of proteins and their concentration in foods, biological fluids, and tissues. Because of the vast number of amino acids, the chromatographic techniques provide separations of them based either on the charge or on hydrophobicity differences. Several chromatographic techniques have been applied for this purpose, with the method of choice being ion-exchange chromatography followed by post-column derivatization, as a more rugged and reproducible method provides excellent amino acid separation with relative freedom from interferences. This review considers recent relative reviews, current separation techniques, the most popular post-column reactions for amino acid analysis, comparison with the pre-column methods, the...
全文获取路径: Taylor & Francis  (合作)
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影响因子:0.429 (2012)

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