NBD-Cl as a Post-Column Reagent for Primary and Secondary Amines after Separation by Ion-Exchange Chromatography
作者: Constantinos K. ZacharisParaskevas D. TzanavarasPanagiotis N. MouroutisPantelis G. Rigas
作者单位: 1Laboratory of Analytical Chemistry, Department of Chemistry , Aristotelian University of Thessaloniki , Thessaloniki , Greece
2Department of Food Technology , School of Food Technology and Nutrition, Alexander Technological Educational Institute (ATEI) of Thessaloniki , Thessaloniki , Greece
3Biotoxins and Analytical Chemistry Laboratory, Department of Fisheries and Aquaculture Technology , Alexander Technological Educational Institute of Thessaloniki , N. Moudania , Greece
刊名: Analytical Letters, 2011, Vol.44 (10), pp.1821-1834
来源数据库: Taylor & Francis Journal
DOI: 10.1080/00032719.2010.526263
关键词: Aliphatic aminesFluorescenceIon-exchange chromatographyPost-column derivatization4-chloro-7-nitrobenzo-2-oxa-13-diazole (NBD-Cl)
原始语种摘要: In this study 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) is proposed as a post-column derivatization (PCD) reagent for the fluorescence detection of aliphatic primary and secondary amines after HPLC separation. Five primary (methylamine, isoamylamine, 2-phenylethylamine, putrescine, and histamine) and one secondary amine (dimethylamine) were separated isocratically on a cation-exchange column using HNO3 (5 × 10−3 mol L−1) as the mobile phase. Post-column derivatization was based on two steps: 1) the derivatization of amines with NBD-Cl in alkaline medium, and 2) the acidification of the resulted mixture in order to minimize the background signal of the reagent and improve dramatically the sensitivity and determination range. The variables of the...
全文获取路径: Taylor & Francis  (合作)
影响因子:0.965 (2012)