Isolation and Characterization of Embryonic Stem Cell-Like Cells From in vitro Produced Goat ( Capra hircus ) Embryos
作者: Arun Kumar DeDhruba MalakarYogesh S. AksheyManoj Kumar JenaRahul Dutta
作者单位: 1Animal Biotechnology Centre , National Dairy Research Institute , Karnal , Haryana , India
刊名: Animal Biotechnology, 2011, Vol.22 (4), pp.181-196
来源数据库: Taylor & Francis Journal
DOI: 10.1080/10495398.2011.622189
关键词: BlastocystsEmbryonic stem cell-like cellsFeeder layerGoatOct-4
原始语种摘要: The aim of the present study was to isolate and characterize goat embryonic stem cell-like cells from in vitro produced goat embryos. Inner cell mass (ICM) cells were isolated either mechanically or by enzymatic digestion from 150 blastocysts and 35 hatched blastocysts whereas 100 morulae were used for blastomeres isolation mechanically. The ICM derived cells or blastomeres were cultured on a feeder layer. The primary colony formation was significantly higher ( P < 0.01) for hatched blastocysts (77.14%) than early/expanded blastocysts (54%) or morula (14%). When ICMs were isolated mechanically the primary colony formation for hatched blastocysts (90%) as well as blastocysts (66%) were significantly more than when ICMs were isolated by enzymatic digestion (60% and 30%, respectively). The...
全文获取路径: Taylor & Francis  (合作)
影响因子:0.882 (2012)