Ion Interaction Chromatographic Separation of Amino Acids Using a Basic-Tetraalkylammonium Salt Mobile Phase
作者: Pantelis G. RigasSteve J. ArvanitisDonald J. Pietrzyk
作者单位: 1Chemistry Department Iowa City, The University of Iowa, Iowa
刊名: Journal of Liquid Chromatography & Related Technologies, 1987, Vol.10 (13), pp.2891-2910
来源数据库: Taylor & Francis Journal
DOI: 10.1080/01483918708066834
原始语种摘要: A basic mobile phase containing a tetraalkylammonium (R4N+) salt was used to enhance the retention of free amino acids (AA) in their anion form on a polystyrene divinylbenzene copolymeric (Hamilton PRP-1) nonpolar stationary phase adsorbent. Major variables, which can be readily manipulated to alter this retention and resolve complex AA mixtures, are: structure and concentration of R4N+ salt, type and amount of organic modifier in the mobile phase solvent, concentration and selectivity of the counteranion present, and mobile phase pH and ionic strength. Mobile phase gradients based on a pH change, or an ionic strength change and their combination, while all other variables are constant, were evaluated for the separation of complex AA mixtures. Detection was accomplished by absorbance or...
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