The prevention of sedentary behavior and discussing the relationship between sedentary behavior and health are very significant. In this study, sEMG was used to detect the changes of spinal muscle activity in three positions: sitting, and standing. It was aimed to find out the sEMGcharacteristics of splenius capitis (SCA, trapezius (TR, erector spine muscle (ESM and spinalis (SP under the sedentary, sitting-moving and sittingstanding posture. Through the two-factor repeated measures analyses of variance, the results revealed that there were a significant differencein MPF and MF between SCA, ESM, TR and SP at different time points. The results showed that the MF;and MPF;of ESM, SP, TR, and SCA were smaller in the sitting position than in the sitting position. The MF;and MPF;of both SP... andSCA were larger than that in the sedentary posture. These results confirm that sitting posture can alleviate spinal muscle fatigue compared to sedentary posture, while sitting posture can alleviate ESM and TR fatigue. The sitting-moving posture can’t improve the fatigue of SCA and SPwhich caused by sedentary posture.