Isolation of Male Sterile and Maintainer Lines from North-Indian Onion (Allium cepa L.) Populations with the Aid of PCR-Based Molecular Marker
作者: Geetika MalikAjmer Singh DhattAjaz Ahmed Malik
刊名: Vegetos- An International Journal of Plant Research, 2017, Vol.30 (2), pp.94-99
来源数据库: Indianjournals.com
DOI: 10.5958/2229-4473.2017.00142.2
关键词: OnionS-cytoplasmMale sterileMaintainerMarker assisted selection
原始语种摘要: Marker assisted selection (MAS) using mitochondrial DNA based marker cytochrome b (cob) was integrated with phenotypic evaluation to isolate male sterile and maintainer lines from open-pollinated onion varieties adapted to North Indian agro-climatic region. Cytotype (N/S) determination by cob marker followed by morphological and microscopic study of pollen discovered male sterile plants ( Smsms ) at frequencies of 0.015 in Punjab Naroya, 0.020 in Punjab Selection, and 0.006 in Punjab White. The progeny scoring of test-crosses between male sterile and N-cytoplasmic plants isolated the maintainers (Nmsms) at frequencies of 0.133 in Punjab Naroya, 0.231 in Punjab Selection and 0.182 in Punjab White. As a novel approach, Trait Recovery Programme was demonstrated to reduce the population size...
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关键词翻译
关键词翻译
  • sterile 无生殖力的
  • maintainer 维修人员
  • cytotype 细胞型
  • population 母体
  • Selection 分选
  • recessive 隐性
  • progeny 后代
  • lines 线型
  • adapted 适合的
  • phenotypic 表型的