In vitro direct plant regeneration protocol for tomato genotypes
作者: Ruma DeviM.S. DhaliwalS.S. Gosal
刊名: Indian Journal of Horticulture, 2013, Vol.70 (3), pp.369-372
关键词: Growth hormonestomatoorganogenesistissue culture
原始语种摘要: A high frequency direct plant regeneration method was developed for four tomato genotypes, viz ., Castle Rock, Punjab Upma, VFN-8 and IPA-3. Cotyledons of 8-day-old in vitro grown seedlings of the four genotypes were excised and cultured onto Murashige an Skoog (MS) medium supplemented with different concentrations and combinations of BAP and kinetin. The frequency of plant regeneration and number of shoots per explant was influenced by the genotype and concentration of growth regulators. Among the genotypes tested, IPA-3 recoded the maximum plant regeneration (95.14%) followed by Punjab Upma (93.13%), Castle Rock (90.02%) and VFN-8 (82.09%). Among the different media compositions tried maximum average plant regeneration (90.14%) and number of shoots (6.17) were obtained using MS medium...
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  • plant 植物
  • regeneration 再生
  • tomato 番茄
  • kinetin 激动素
  • shoot 萌蘖
  • glasshouse 温室
  • elongation 伸长
  • different 不相同的
  • direct 直接的
  • concentration 浓度