An experiment was carried out during 2017–18 at Department of Horticulture, School of Agricultural Sciences, District Dehradun, Uttarakhand to see the effect of picking dates on physico-chemical characteristics of Karonda cultivars. The treatments comprised three different dates of picking of berries (40, 60 and 80 Days after fruit set) and three cultivars viz Pant Manohar, Pant Sudarshan and Pant Suvarna. All the 9 treatment combinations were laid out in a 3 x 3 factorial randomized block design with 3 replications. Observations were recorded at three different picking dates. The treatment combination C3D3 (Pant Suvarna + 80 Days after fruit set) was found better in terms of moisture, total soluble solids, reducing sugar, non-reducing sugar, total sugar, titratable... acidity, ascorbic acid, phosphorous, calcium and iron content i.e. maximum moisture content (89.41%), highest TSS (8.53°Brix), highest reducing sugar (6.20%), highest non-reducing sugar (2.03%), highest total sugar (8.33%), highest iron (6.74 mg/100g), maximum ascorbic acid (12.06 mg/100g) and lowest titratable acidity (1.74%), followed by C1D3 (Pant Manohar + 80 Days after fruit set) and C2D3 (Pant Sudarshan + 80 Days after fruit set). The treatment combination C3D2 (Pant Suvarna + 60 Days after fruit set) recorded maximum pectin content.