Cholesterol Loaded Cyclodextrin Increases cryopreservability of Marwari Stallion ( Equus ferus caballus ) Spermatozoa by Increasing Cholesterol to Phospholipid Ratio
作者: Pramod KumarJ.S. MehtaS.K. RaviTalluri T RaoG.N. PurohitAshok Kumar Chaudhary
刊名: Journal of Animal Research, 2019, Vol.9 (2), pp.227-235
DOI: 10.30954/2277-940X.02.2019.2
关键词: Marwari stallionCholesterol loaded cyclodextrin (CLC)CholesterolPhospholipidCholesterolPhospholipid (CP) ratio
原始语种摘要: The study was conducted to investigate the effects of different levels of cholesterol loaded cyclodextrin (CLC) addition on cooled and frozen-thawed spermatozoa of Marwari stallion. A total of 48 ejaculates were collected from six adult Marwari stallions (8 ejaculates from each stallion) aged between 4 to 7 years. Immediately after collection semen sample was macroscopically evaluated and filtered into a warm, graduated measuring bottle to get gel free semen. The level of cholesterol (C) and phospholipid (P) in fresh spermatozoa were measured using ELISA. The semen sample was divided in to five equal aliquots (T0, T1, T2, T3 and T4). Primary extender containing different concentrations of CLC was added to each aliquot (0, 1,...
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  • spermatozoa 精子
  • stallion 公马
  • semen 精液
  • loaded 加负荷的
  • cholesterol 胆甾醇
  • phospholipid 磷脂
  • ratio 
  • cyclodextrin 环糊精
  • graduated 有刻度的
  • freeze