Objectives: Increasing cardiovascular patients is a matter of global issue. Various drugs are being used to cure these ailments with different mode of actions. The present article describes simultaneous and Fast SPE-HPLC analyses of nine anti-hypertensive drugs in human plasma.Methods: Cardiovascular drugs such as amiloride, metoprolol, hydrochlorothiazide, carvidilol, amlodipine, fusemide, telmisertan, losartan and olmesartan were separated by HPLC on Sushell C8 (150 x 4.6 mm, 2.6 µm) column. The mobile phase was phosphate buffer (65% with 0.8% TEA) in acetonitrile; 0.5 mL/min flow rate, 230 nm detection and 40±1ºC temperature.Results: The values of retention, separation and resolution factors were from 0.19-3.40, 1.20-3.60 and 2.43-12.37, respectively. The values of retention times,... tailing factor and number of theoretical plates ranged from 2.64-11.60, 1.20-1.34 and 25883-144283, respectively. The percentage bindings with plasma determined by SPE were 40, 35, 70, 90, 70, 90, 90, 90, and 90%, respectively. The developed SPE-HPLC methods were applied successfully for monitoring these drugs into human plasma.