Pallavi A. Patil, A.R. Umarkar, M.M. Bari, S.D. Barhate
||Open Journal of Advanced Drug Delivery, 2015, Vol.3 (2), pp.110-122
||Pubicon International Publications
Objective: A Stability indicating Reverse-Phase liquid chromatographic method for the simultaneous estimation of Drotaverine HCl and Mefenamic acid was developed.Method: The chromatographic assay involves the use of Hi Q C-18 W (5µm column with a simple mobile phase containing 80:20% v/v of methanol & HPLC Grade water (0.1% triethylamine at pH 3) with flow rate of 1.0mL/min with UV detection at wavelength of 250 nm. Result: The method showed good linearity in the concentration range of 2-10 μg/mL for Drotaverine Hcl and 6-30 μg/mL for Mefenamic Acid.Conclusion: The developed method was successfully validated as per the ICH guidelines. The stability indicating capacity was also tested and successfully applied to marketed formulation.