Simultaneous determination of warfarin and 7-hydroxywarfarin in rat plasma by HPLC-FLD
作者: Aref ZayedWahby M. BabareshRuba S. DarweeshTamam El-Elimat
作者单位: 1Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy , Jordan University of Science and Technology , Irbid 22110 , Jordan
2 Department of Pharmaceutical Technology, Faculty of Pharmacy , Jordan University of Science and Technology , Irbid 22110 , Jordan
刊名: Acta Pharmaceutica, 2020, Vol.70 (3), pp.343-357
来源数据库: De Gruyter Journal
DOI: 10.2478/acph-2020-0025
关键词: Warfarin7-hydroxywarfarinHigh-performance liquid chromatography-fluorescence detectionRat plasmaPharmacokinetics
原始语种摘要: Abstract In this study, high-performance liquid chromatography with fluorescence detection (HPLC-FLD) has been used for the first time, for direct determination of warfarin and its major metabolite, 7-hydroxywarfarin, in rat plasma. The simple and sensitive method was developed using Fortis® reversed-phase diphenyl column (150 × 4.6 mm, 3 μm) and a mobile phase composed of phosphate buffer (25 mmol L−1)/methanol/acetonitrile (70:20:10, V / V / V ), adjusted to pH 7.4, at a flow rate of 0.8 mL min−1. The diphenyl chemistry of the stationary phase provided a unique selectivity for separating the structurally related aromatic analytes, warfarin and 7-hydroxywarfarin, allowing their successful quantification in the complex plasma matrix. The method was linear...
全文获取路径: De Gruyter 
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关键词翻译
关键词翻译
  • warfarin 杀鼠灵
  • plasma 深绿玉髓
  • determination 测定
  • metabolite 蚀变粗玻岩
  • diphenyl 联二苯
  • reversed 倒转的
  • FLD 场地
  • fluorescence 荧光
  • liquid 液体
  • demonstrated 探明的储量