HPTLC determination of diosgenin in fenugreek seeds
作者: Barbara Król-KogusKhenifi Mohammed LaminePiotr MigasMessaoud BoudjenibaMirosława Krauze-Baranowska
作者单位: 11 Department of Pharmacognosy with Medicinal Plants Garden Medical University of Gdańsk 80-416 Gdańsk , Poland
22 Laboratoire d’Ethnobotanique et Substances Naturelles Ecole Normale Supérieure de Kouba B.P 92 Kouba , 16050 Algerie
刊名: Acta Pharmaceutica, 2018, Vol.68 (1), pp.97-107
来源数据库: De Gruyter Journal
DOI: 10.2478/acph-2018-0002
关键词: Fenugreek (Trigonella foenum-graecum)DiosgeninHPTLCQuantitative analysis
原始语种摘要: Abstract A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L-1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent. The method was preliminarily validated and the determined amounts of diosgenin in fenugreek seeds of Polish and African origin were found to be similar and ranged from 0.12-0.18 %.
全文获取路径: De Gruyter 

  • diosgenin 薯蓓甙元
  • fenugreek 胡芦巴
  • HPTLC 高效薄层色谱法
  • determination 测定
  • anisaldehyde 茴香醛
  • extraction 提取
  • saponin 皂荚苷
  • hydrolysis 水解
  • reagent 试剂
  • spraying 喷涂