TWO NOVEL MYCOVIRUSES RELATED TO GEMINIVIRUS ISOLATED FROM THE SOIL-BORNE FUNGI MACROPHOMINA PHASEOLINA (TASSI) GOID. AND MUCOR RACEMOSUS BULL.
作者: Elsayed Elsayed HafezDalia Gamil AseelSaad Mostafa
作者单位: 1City of Scientific Research and Technology Applications, Arid Lands Cultivation Research Institute, Alexandria, Egypt
刊名: Biotechnology & Biotechnological Equipment, 2013, Vol.27 (6), pp.4222-4226
来源数据库: Diagnosis Press Journal
DOI: 10.5504/BBEQ.2013.0086
关键词: mycovirusesFusarium oxysporumssDNAvirus-like particlesviral transmission
原始语种摘要: Viral coat protein gene was detected in thirty isolates of different soil-borne fungi, using cotton leaf curl coat protein specific primers (geminiviruses). Out of them, seven fungal isolates showed positive results (a band with a molecular size of 680 bp). The products amplified from two isolates, Macrophomina phaseolina (Tassi) Goid. and Mucor racemosus Bull., were excised from the gel, purified and sequenced. The sequence analysis revealed that the amplicons were coat protein genes of geminivirus. The gene isolated from M. phaseolina was similar to that of wheat dwarf geminivirus with identity not exceeding 36 %; and the coat protein isolated from M. racemosus was 43 % identical with the maize streak geminivirus. These low identities suggest that the amplification products belong to...
全文获取路径: Diagnosis Press 
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关键词翻译
关键词翻译
  • geminivirus 双生病毒
  • observed 观察到的
  • amplified verification扩大核查
  • isolated 隔离
  • purified 净化的
  • protein 蛋白质
  • ssDNA 单链 DNA
  • evolution 进化
  • investigate 
  • mycelia 菌丝体