Zinc Chelation Mediates the Lysosomal Disruption without Intracellular ROS Generation
作者: Andreza Cândido MatiasTânia Maria ManieriGiselle CerchiaroTetsuro Kamiya
作者单位: 1Center for Natural Sciences and Humanities, Federal University of ABC, UFABC, Avenida dos Estados 5001, Bloco B, 09210-170 Santo André, SP, Brazil
刊名: Oxidative Medicine and Cellular Longevity, 2016, Vol.2016
来源数据库: Hindawi Journal
DOI: 10.1155/2016/6724585
原始语种摘要: We report the molecular mechanism for zinc depletion caused by TPEN (N,N,N′,N′-Tetrakis(2-pyridylmethyl)ethylenediamine) in neuroblastoma cells. The activation of p38 MAP kinase and subsequently caspase 3 is not due to or followed by redox imbalance or ROS generation, though these are commonly observed in literature. We found that TPEN is not responsible for ROS generation and the mechanism involves essentially lysosomal disruption caused by intracellular zinc depletion. We also observed a modest activation of Bax and no changes in the Bcl-2 proteins. As a result, we suggest that TPEN causes intracellular zinc depletion which can influence the breakdown of lysosomes and cell death without ROS generation.
全文获取路径: Hindawi 
影响因子:3.393 (2012)

  • ROS Read Only Storage
  • responsible 负责的
  • generation 世代
  • lysosomes 溶菌体
  • depletion 矿量递减
  • activation 活化
  • neuroblastoma 成神经细胞瘤
  • disruption 破裂
  • breakdown 故障
  • intracellular 胞内的