FACTORS AFFECTING LIQUID CHROMATOGRAPHY TANDEM-MASS SPECTROMETRY ANALYSIS OF 6-MERCAPTOPURINE AND 6-THIOGUANINE IN DRIED BLOOD SPOTS
作者: Yahdiana HarahapDimas Agus Putera HardijantoDelly Ramadon
作者单位: 1Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.. yahdiana03@yahoo.com
刊名: International Journal of Applied Pharmaceutics, 2018, Vol.10 (1), pp.200-202
来源数据库: Innovare Academics Sciences PVT.Ltd.
DOI: 10.22159/ijap.2018.v10s1.44
原始语种摘要: Objective: This study aimed to determine the effects of the method of internal standard addition, spotting volume, paper type, and sample storagetemperature on 6-mercaptopurine, and 6-thioguanine on liquid chromatography tandem-mass spectrometry (LC-MS/MS) bioanalysis methods usingdried blood spot (DBS).Methods: Blood samples were spotted on CAMAG DBS paper and a Perkin Elmer 226 sample collection device (paper) and extracted into methanolcontaining 5-fluorouracil as an internal standard. The separation was performed on a water acquity ultra high-performance LC BEH Amide 1.7 μm(2.1 mm×100 mm) column with a mobile phase of 0.2% formic acid in water - 0.1% formic acid in acetonitrile methanol with gradient elution at aflow rate of 0.2 mL/min.Results: The step at which the internal standard...
全文获取路径: IAS 
分享到:

×
关键词翻译
关键词翻译
  • affected 受了影响的
  • paper 
  • bioanalysis 生物分析法
  • determine 决心
  • whereas 鉴于
  • internal 内部的
  • standard 标准
  • thioguanine 硫鸟嘌呤
  • blood 血液
  • acetonitrile 乙腈