The Development of a High Density Linkage Map for Black Tiger Shrimp ( Penaeus monodon ) Based on cSNPs
作者: Matthew BaranskiGopalapillay GopikrishnaNicholas A. RobinsonVinaya Kumar KatneniMudagandur S. ShekharJayakani ShanmugakarthikSarangapani JothivelChavali GopalPitchaiyappan RavichandranMatthew KentMariann ArnyasiAlphis G. Ponniah
作者单位: 11 Nofima, Ås, Norway
2 2 Central Institute of Brackishwater Aquaculture, Raja Annamalai Puram, Chennai, Tamil Nadu, India
3 3 Flinders University, Bedford Park, South Australia, Australia
4 4 Centre for Integrative Genetics (CIGENE), Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Ås, Norway
刊名: PLOS ONE, 2014, Vol.9 (1)
来源数据库: PLOS
DOI: 10.1371/journal.pone.0085413
原始语种摘要: Transcriptome sequencing using Illumina RNA-seq was performed on populations of black tiger shrimp from India. Samples were collected from (i) four landing centres around the east coastline (EC) of India, (ii) survivors of a severe WSSV infection during pond culture (SUR) and (iii) the Andaman Islands (AI) in the Bay of Bengal. Equal quantities of purified total RNA from homogenates of hepatopancreas, muscle, nervous tissue, intestinal tract, heart, gonad, gills, pleopod and lymphoid organs were combined to create AI, EC and SUR pools for RNA sequencing. De novo transcriptome assembly resulted in 136,223 contigs (minimum size 100 base pairs, bp) with a total length 61 Mb, an average length of 446 bp and an average coverage of 163× across all pools. Approximately 16% of contigs were...
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影响因子:3.73 (2012)

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关键词翻译
关键词翻译
  • sequencing 排序
  • length 距离
  • female 女性的
  • purified 净化的
  • organs 男生殖器
  • offspring 后代
  • observed 观察到的
  • marker 标试器
  • RNA ROYAL NEPAL AIRLINES CORP.
  • average 平均