Heme Oxygenase-1 Regulates Matrix Metalloproteinase MMP-1 Secretion and Chondrocyte Cell Death via Nox4 NADPH Oxidase Activity in Chondrocytes
作者: Francis RoussetMinh Vu Chuong NguyenLaurent GrangeFrançoise MorelBernard Lardy
作者单位: 11 Université Joseph Fourier, AGIM GREPI FRE CNRS 3405, Grenoble, France
2 2 Rheumatology Department, Grenoble Teaching Hospital, CHU Hôpital Sud, Grenoble, France
3 3 Laboratoire de Biochimie Enzyme et Protéine-DBTP, Institut de Biologie et de Pathologie, Centre Hospitalier Universitaire (CHU), Grenoble, France
刊名: PLOS ONE, 2013, Vol.8 (6)
来源数据库: PLOS
DOI: 10.1371/journal.pone.0066478
原始语种摘要: Interleukin-1β (IL-1β) activates the production of reactive oxygen species (ROS) and secretion of MMPs as well as chondrocyte apoptosis. Those events lead to matrix breakdown and are key features of osteoarthritis (OA). We confirmed that in human C-20/A4 chondrocytes the NADPH oxidase Nox4 is the main source of ROS upon IL-1β stimulation. Since heme molecules are essential for the NADPH oxidase maturation and activity, we therefore investigated the consequences of the modulation of Heme oxygenase-1 (HO-1), the limiting enzyme in heme catabolism, on the IL-1β signaling pathway and more specifically on Nox4 activity. Induction of HO-1 expression decreased dramatically Nox4 activity in C-20/A4 and HEK293 T-REx™ Nox4 cell lines. Unexpectedly, this decrease was not accompanied by any change in...
全文获取路径: PLOS 
影响因子:3.73 (2012)

  • NADPH 还原型烟酰胺腺嘌呤二核苷酸磷酸
  • oxygenase 氧合酶
  • maturation 成熟
  • osteoarthritis 骨关节炎
  • stimulation 激励
  • expression 表示
  • secretion 分泌
  • activity 活度
  • heterodimer 异质二聚体
  • catabolism 分化酌