Stable archaeal tetraether lipid liposomes for photodynamic application: transfer of carboxyfluorescein to cultured T84 tumor cells
作者: Anton OertlEmmanouil AntonopoulosSeruni U. FreislebenHans-Joachim Freisleben
作者单位: 1Faculty of Medicine, Johann-Wolfgang-Goethe University, Frankfurt am Main
2Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok
刊名: Medical Journal of Indonesia, 2017, Vol.25 (4), pp.196-206
来源数据库: Medical Journal of Indonesia
DOI: 10.13181/mji.v25i4.1506
原始语种摘要: Background: Archaeal membranes have phytanyl ether lipids instead of common fatty acid-glycerol esters in bacterial and eukaryotic cells. Sulfolobus and Thermoplasma species have unique membrane-spanning tetraether lipids (TEL), which form stable liposomes. Recently, we cultured Thermoplasma species from the Indonesian volcano Tangkuban Perahu and isolated TEL. The purpose of this in vitro study is to investigate the transfer of fluorescent dye from stable TEL liposomes to cultured colon carcinoma cells. Methods: TEL was extracted from cultured cells with chloroform-methanol (1:1), then it was fractionated and purified via diethylaminoethyl-cellulose-acetate columns and activated charcoal for the formation of stable liposomes. For the fluorescence exchange assay, TEL liposomes were loaded...
全文获取路径: Medical Journal of Indonesia 
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关键词翻译
关键词翻译
  • tetraether 四醚
  • cultured 人工培养的
  • cells 麻风细胞
  • fluorescent 萤光的
  • photodynamic 在光中发荧光的
  • lipid 脂质
  • carcinoma 癌症
  • stable 稳定的
  • colon 结肠
  • stability 稳定性