The complication of diabetic cardiomyopathy (DCM) is one ofthe major causes of death in diabetes mellitus (DM)patients. Accordingly, an effective animal model is playingan important role in investigating the pathogenesis of DCM.In this study, we randomized Sprague-Dawley (SD) rats into acontrol group and DCM group. The DCM rats were induced withintraperitoneal streptozotocin (STZ) injection. Eight weeksafter STZ injection, compared with the Control rats, the DCMrats exhibited a series of symptoms such as polydipsia,polyphagia, polyuria, weakness, hyperglycemia andemaciation. Echocardiography results revealed that the DCMrats had a left lower ventricular ejection fraction (LVEF),fractional shortening (FS) and E wave to A wave ratio (E/A)ratio than the Control rats. The results... ofhematoxylin-eosin (HE) staining showed that the leftventricle myocytes of the DCM rats were disordered and theircytoplasm was stained unevenly and their nucleus wasirregular compared with the Control rats. Subsequently, asdetected by transmission electron microscopy, compared withthe Control rats, the apical tissue of heart displayeddisorganized myofibrils and fragmented, vacuolated andswollen mitochondria in the DCM rats. Therefore, the DCMrats model were successfully induced via injectingintraperitoneally with a single STZ (65mg/kg).