Detection of clostridium difficile antigen and toxin in stool specimens: Comparison of the C. difficile quik chek complete enzyme immunoassay and GeneXpert C. difficile polymerase chain reaction assay
作者: Abiola SenokKamel AldosariRayan AlowaisheqOthman AbidKhalid AlsuhaibaniMohammad KhanAli Somily
作者单位: 1Department of Basic Science, College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai
2Department of Laboratory Medicine, Microbiology Laboratory, Prince Mohammed Bin Abdulaziz Hospital, Riyadh
3College of Medicine, King Saud University, Riyadh
4Department of Pathology and Laboratory Medicine, College of Medicine, King Saud University and King Saud University Medical City, Riyadh
刊名: Saudi Journal of Gastroenterology, 2017, Vol.23 (4), pp.259-262
来源数据库: Wolters Kluwer Journal
DOI: 10.4103/sjg.SJG_80_17
关键词: Clostridium difficileenzyme immunoassayGeneXpert PCR assay
原始语种摘要: Background/Aims: Accurate and rapid laboratory diagnosis of Clostridium difficile infections (CDI) remains a significant challenge. A two-step algorithm for detection of toxigenic C. difficile in stool based on initial screening for glutamate dehydrogenase assay followed by confirmation by toxin A+B detection using an enzyme immunoassay (EIA) or molecular assay has been proposed. We aimed to evaluate the C. difficile Quik Chek Complete® (QCC-EIA) versus the GeneXpert® C. difficile polymerase chain reaction (PCR) assay in this two-step algorithm.Materials and Methods: Two hundred and ten liquid stool samples obtained between June 2014 and June 2015 from patients suspected of CDI were tested by the QCC-EIA and GeneXpert PCR assay. The GeneXpert assay was used as the...
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  • immunoassay 免疫测定法
  • polymerase 聚合酶
  • stool 
  • EIA Electronic Industries Association
  • clostridium 梭状芽孢杆菌
  • toxin 
  • reaction 反酌
  • screening 筛分
  • chain 
  • QCC Quality Control Center