Inhibition of the Inositol Requiring Protein 1α- X-Box Binding Protein-1 Pathway as a Promising Therapeutic Target for Human Prostate Cancer
作者: M. Khalil AhmadY. Alghadi AhmadM. T. Shahen RahafW. Elasad JehadI. Jawasreh Khaleel
作者单位: 1Department of Biological Sciences, Yarmouk University, Irbid, Jordan
2Department of Applied Biology, Jordan University of Science and Technology, Irbid, Jordan
3Department of Animal Productions, Jordan University of Science and Technology, Irbid, Jordan
刊名: Journal of Cancer Research and Treatment, 2018, Vol.6 (3), pp.74-79
来源数据库: Science and Education Publishing
DOI: 10.12691/jcrt-6-3-3
原始语种摘要: Prostate cancer (PCa) has been associated with endoplasmic reticulum stress (ERS) which activates the inositol requiring protein 1α- X-box binding protein-1 (IREα-XBP-1) pathway. The aim of the study was to investigate the role of this pathway in three human PCa cell lines (LNCaP, PC-3, and DU-145) by evaluating the expression of XBP-1 and glucose-regulated protein 78 (GRP78) genes. The effect of two ERS inducers (Thapsigargin, Tg and tunicamycin, Tm) alone and in combination with an inhibitor of the IRE1α RNase inhibitor (STF-083010) on expression profiling was followed using Quantitative-PCR. In vitro treatment of PCa cells with ERS inducers upregulated expression of XBP-1 gene. STF-083010 inhibited IRE1α-induced splicing of the gene and increased cytotoxicity. Inhibition of IRE1α RNase...
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  • Inositol 环己六醇
  • chaperon 唇基
  • protein 蛋白质
  • inositol 肌醇
  • pathway 轨道
  • expression 表示
  • splicing 接合
  • alone 单独地
  • targeting 导向目标
  • Cancer 巨蟹座