Effect of nitric oxide on VnfA, a transcriptional activator of VFe-nitrogenase in Azotobacter vinelandii
作者: Yukio MiuraKyohei YoshimitsuNobuyuki TakataniYoshihito WatanabeHiroshi Nakajima
作者单位: 11Department of Chemistry, Graduate School of Science; 2Graduate School of Bioagricultural Science; and 3Research Center of Materials Science, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya 464-8602, Japan
刊名: Journal of Biochemistry, 2015, Vol.157 (5), pp.365-375
来源数据库: Duke University Press
DOI: 10.1093/jb/mvu083
关键词: dinitrosyl–ironcomplexironsulphurclusternitricoxidenitrogenasetranscriptional activator
原始语种摘要: The transcriptional activator, VnfA, is necessary for the expression of the structural genes encoding vanadium-dependent nitrogenase in Azotobacter vinelandii. We have previously reported that VnfA harbours a Fe-S cluster as a prosthetic group, presumably a 3Fe-4S type, which is vital for the transcriptionally active VnfA. A plausible effector molecule is a reactive oxygen species (ROS), which disassembles the Fe-S cluster switching the active VnfA to become fully inactive. This finding prompted us to investigate the effect of nitric oxide (NO), another physiologically important radical species on the VnfA activity. Unlike ROS, the VnfA activity was moderately inhibited and converged to 70% of the maximum by NO irrespective of its concentration. The Fe-S cluster of VnfA was found to react...
全文获取路径: Duke University Press 
影响因子:2.719 (2012)

  • active 活动的
  • effector 效应器效应物
  • nitric 氮的
  • relative 相对的
  • concentration 浓度
  • investigate 
  • fully 充分
  • cluster 
  • activator 活化剂
  • expression 表示