Identification of a Cyanobacterial RND-Type Efflux System Involved in Export of Free Fatty Acids
作者: Akihiro KatoNobuyuki TakataniKazuhide UseKazuma UesakaKazutaka IkedaYajun ChangKouji KojimaMakiko AichiKunio IharaKenji NakahigashiShin-ichi MaedaTatsuo Omata
作者单位: 11Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan
22Institute for Advanced Biosciences, Keio University, Yamagata, 997-0052 Japan
33Department of Biological Chemistry, Chubu University, Kasugai, 487-8501 Japan
44Center for Gene Research, Nagoya University, Nagoya, 464-8602 Japan
55Japan Science and Technology Agency, CREST
66Present address: Laboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences, Yokohama, 230-0045 Japan.
77Present address: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, Jiangsu, PR China.
88Present address: Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan.
刊名: Plant and Cell Physiology, 2015, Vol.56 (12), pp.2467-2477
来源数据库: Duke University Press
DOI: 10.1093/pcp/pcv150
关键词: Biofuel productionCyanobacteriaFree fatty acidRND-type efflux system
原始语种摘要: An RND (resistance–nodulation–division)-type transporter having the capacity to export free fatty acids (FFAs) was identified in the cyanobacterium Synechococcus elongatus strain PCC 7942 during characterization of a mutant strain engineered to produce FFAs. The basic strategy for construction of the FFA-producing mutant was a commonly used one, involving inactivation of the endogenous acyl-acyl carrier protein synthetase gene (aas) and introduction of a foreign thioesterase gene (‘tesA), but a nitrate transport mutant NA3 was used as the parental strain to achieve slow, nitrate-limited growth in batch cultures. Also, a nitrogen-regulated promoter PnirA was used to drive ‘tesA to maximize thioesterase expression during the nitrate-limited growth. The resulting mutant (dAS2T) was, however,...
全文获取路径: Duke University Press 
影响因子:4.134 (2012)

  • nitrate 硝酸盐
  • growth 生长
  • mutant 突变体
  • strain 应变
  • encode 编成密码
  • efflux 
  • permissive 许可的
  • maximize 使达到最大限度
  • expression 表示
  • demonstrate 说明