New Transcriptional Reporters to Quantify and Monitor PPAR γ Activity
作者: Séverine A. DegrelleHussein ShoaitoThierry FournierConstantinos Giaginis
作者单位: 1INSERM, UMR-S1139, Faculté de Pharmacie de Paris, 75006 Paris, France
2Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France
3Fondation PremUp, 75006 Paris, France
刊名: PPAR Research, 2017, Vol.2017
来源数据库: Hindawi Journal
DOI: 10.1155/2017/6139107
原始语种摘要: The peroxisome-proliferator-activated-receptor- γ (PPAR γ ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPAR γ , we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPAR γ activity in different cell types and screen for PPAR γ ’s potential ligands.
全文获取路径: Hindawi 
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影响因子:2.685 (2012)

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关键词翻译
关键词翻译
  • Activity 放射性活度
  • superfamily 总科
  • fluorescent 萤光的
  • receptor 接受体
  • reporter 报告生成程序
  • adipogenesis 脂肪生成
  • potential 
  • metabolism 代谢
  • peroxisome 过氧化物酶体
  • histone 组蛋白