Aberrant expression of miR-127 in rheumatoid arthritis and its related mechanism
作者: Xuqiang Wu* Weijie Gu Bing Zheng
刊名: Advances in biology and medicine, 2018, Vol.3 (4)
来源数据库: Hong Kong Heptaviolet Cultural Publishing Co., Limited
关键词: MiR-127Rheumatoid arthritisOsteopontin
原始语种摘要: Abstract Objective: This study aims to investigate the role and mechanism of miR-127 in rheumatoid arthritis (RA). Methods: Totally 30 cases of RA patients were enrolled and 30 cases of healthy volunteers were enrolled as the control group. Peripheral blood samples and synovial tissues of the participators were collected, and RT-qPCR has been performed to detect the expression of miR-127; moreover, fibroblast-like synoviocytes (SFs) of patients with RA were cultured and stimulated with TNF-α, and transfected with miR-127 mimics, and MTT assay as well flow cytometry assay have been performed to examine the effect of miR-127 on the proliferation and apoptosis of SFs; furthermore, cells were collected, and RT-qPCR as well as western blot assays have been performed to examine the expression...
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关键词翻译
关键词翻译
  • expression 表示
  • arthritis 关节炎
  • mechanism 机构
  • rheumatoid 患风湿病的
  • related 有关的
  • transfection 转染
  • examine 
  • luciferase 荧光素酶
  • increased 增加
  • furthermore 此外