Abstract Non-small cell lung cancer (NSCLC) accounts for more than 80% of lung cancer and the high mortality probably be caused by early invasion and metastasis. LncRNA FOXD2-AS1 is involved in tumor proliferation, invasion and metastasis. Epithelial-to-mesenchymal transition (EMT) is the main critical process of progression and metastasis. Therefore, the aim of the study is to investigate the expression of FOXD2-AS1 in NSCLC and identify the effects of FOXD2-AS1 on EMT in lung cancer. The results showed that the expression of FOXD2-AS1 was significantly upregulated in NSCLC, and over-expression of FOXD2-AS1 promoted cell migration and invasion in lung cancer cells. In addition, over-expression of FOXD2-AS1 regulated EMT-related molecules, for instance, the mRNA and protein expression... levels of CK18, α-SMA and FN were increased by FOXD2-AS1, while mRNA and protein expression levels of E-cadherin were decreased by FOXD2-AS1. In conclusion, FOXD2-AS1 promotes the invasion and metastasis of NSCLC, which might be a therapeutic target for the treatment of NSCLC.