miR-183 as a prognostic marker in intervertebral disc degeneration and its role in the proliferation and apoptosis of nucleus pulposus
作者: Peidong Qing Yun Cao* Jianfeng Xu Haitao Deng Mingfan Li
刊名: Advances in biology and medicine, 2019, Vol.4 (1)
来源数据库: Hong Kong Heptaviolet Cultural Publishing Co., Limited
关键词: MiR-183PrognosisIDDProliferationApoptosis
原始语种摘要: Abstract Background Accumulating evidence indicated that microRNAs are involved in the pathogenesis of intervertebral disc degeneration (IDD). The roles of miR-183 in IDD have not been elucidated. Methods A total number of 60 patients with IDD were included in this study, and degenerative nucleus pulposus (NP) tissues of the patients were collected. Total RNA was extracted from NP of patients, and the expression levels of miR-183 were examined using real-time quantitative PCR methods. Next, human NP cells were cultured, and transfected with miR-183 mimics and inhibitors, and the roles of miR-183 on the proliferation and apoptosis of cells were determined using MTT and flow cytometry methods; moreover, the expression of MMP9, Caspase-3, BAX, and BCL-2 were examined using RT-qPCR and...
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  • moreover 此外
  • marker 标试器
  • expression 表示
  • nucleus 晶核
  • intervertebral 椎间的
  • transfection 转染
  • regulate 控制
  • proliferation 增生
  • prognosis 预报