Extraction and processing of high quality RNA from impalpable and macroscopicallyinvisible prostate cancer for microarray gene expression analysis
作者: Thorsten SchlommAndreas LuebkeHolger SültmannOlaf HellwinkelUlrich SauerAnnemarie PoustkaKerstin DavidFelix ChunAlexander HaeseMarkus GraefenAndreas ErbersdoblerHartwig Huland
作者单位: 1Department of Urology, University Clinic Hamburg-Eppendorf,D-20246 Hamburg, Germany. tschlomm@uke.uni-hamburg.de
刊名: International Journal of Oncology, 2005, Vol.27 (3), pp.713-720
来源数据库: Spandidos Journal
原始语种摘要: Molecular analyses of early-stage prostate cancers are necessary to assesstheir potential clinical significance based on established and/or novel biomarkersfor tailored clinical management. A prerequisite for the application of RNA-basedanalyses of such, mostly macroscopically-undetectable, small prostate carcinomasis the recovery and preservation of sufficient RNA quantities and quality. Furthermore,in prostate cancer, heterogeneity is a common phenomenon that includes a juxtapositionof different tissue compositions and variable histological grades within the sametumor focus. To better understand the molecular mechanisms of prostate cancer,it is essential to correlate molecular data with a specific cell type. Here, wepresent a tissue collecting protocol which is aligned with the...
全文获取路径: Spandidos 
影响因子:2.657 (2012)

  • prostate 前列腺
  • expression 表示
  • amplified verification扩大核查
  • quality 品质
  • small 小的
  • regulated 规定
  • undetectable 不易发现的
  • cDNA 互补 DNA
  • preoperative 操作前的